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dylight 549 conjugated streptavidin  (Vector Laboratories)


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    Vector Laboratories dylight 549 conjugated streptavidin
    Double fluorescence labeling of HEK293T cells transiently expressing the CB1 receptor using an anti-CB1 antibody and the biotinylated linear polymer fabricated here. Cells were colabeled with a rabbit polyclonal antibody targeting the extracellular N-terminal domain of the human CB 1 receptor and a biotinylated linear polymer specific for the last 12 amino acids of its intracellular C-terminal tail. Detection was performed using Alexa Fluor 488-conjugated antirabbit IgG (green) and Alexa <t>Fluor</t> <t>549-conjugated</t> streptavidin (red). Cell nuclei were counterstained with Hoechst 33342 (blue). (A–F). Cells were fixed and permeabilized prior to simultaneous incubation with both the antibody and the linear polymer, allowing access to both surface and intracellular epitopes. (G–L). Cells were first incubated live with the N-terminal antibody, then fixed and permeabilized before incubation with the linear polymer. This approach restricted antibody binding to surface-exposed domains while permitting the polymer to access both compartments. CB 1 receptor-overexpressing cells (solid arrows) displayed strong signals for both probes, whereas nontransfected cells (open arrows) showed low or negligible labeling with the biotinylated linear polymer, supporting its specificity for the CB 1 receptor C-terminal region. Images correspond to maximum intensity projections of six consecutive optical sections (0.24 μm apart), acquired using structured illumination (ApoTome) on a Carl Zeiss Axio Observer microscope equipped with a motorized XYZ stage. Scale bar = 10 μm (applies to A–L).
    Dylight 549 Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 9457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 549 conjugated streptavidin/product/Vector Laboratories
    Average 99 stars, based on 9457 article reviews
    dylight 549 conjugated streptavidin - by Bioz Stars, 2026-03
    99/100 stars

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    1) Product Images from "Controlled Photoiniferter RAFT-Based Development of Linear Polymers Induced by the Presence of a Peptide to Produce Synthetic Antibody Substitutes Applicable to Immunofluorescence Imaging Techniques"

    Article Title: Controlled Photoiniferter RAFT-Based Development of Linear Polymers Induced by the Presence of a Peptide to Produce Synthetic Antibody Substitutes Applicable to Immunofluorescence Imaging Techniques

    Journal: Analytical Chemistry

    doi: 10.1021/acs.analchem.5c05598

    Double fluorescence labeling of HEK293T cells transiently expressing the CB1 receptor using an anti-CB1 antibody and the biotinylated linear polymer fabricated here. Cells were colabeled with a rabbit polyclonal antibody targeting the extracellular N-terminal domain of the human CB 1 receptor and a biotinylated linear polymer specific for the last 12 amino acids of its intracellular C-terminal tail. Detection was performed using Alexa Fluor 488-conjugated antirabbit IgG (green) and Alexa Fluor 549-conjugated streptavidin (red). Cell nuclei were counterstained with Hoechst 33342 (blue). (A–F). Cells were fixed and permeabilized prior to simultaneous incubation with both the antibody and the linear polymer, allowing access to both surface and intracellular epitopes. (G–L). Cells were first incubated live with the N-terminal antibody, then fixed and permeabilized before incubation with the linear polymer. This approach restricted antibody binding to surface-exposed domains while permitting the polymer to access both compartments. CB 1 receptor-overexpressing cells (solid arrows) displayed strong signals for both probes, whereas nontransfected cells (open arrows) showed low or negligible labeling with the biotinylated linear polymer, supporting its specificity for the CB 1 receptor C-terminal region. Images correspond to maximum intensity projections of six consecutive optical sections (0.24 μm apart), acquired using structured illumination (ApoTome) on a Carl Zeiss Axio Observer microscope equipped with a motorized XYZ stage. Scale bar = 10 μm (applies to A–L).
    Figure Legend Snippet: Double fluorescence labeling of HEK293T cells transiently expressing the CB1 receptor using an anti-CB1 antibody and the biotinylated linear polymer fabricated here. Cells were colabeled with a rabbit polyclonal antibody targeting the extracellular N-terminal domain of the human CB 1 receptor and a biotinylated linear polymer specific for the last 12 amino acids of its intracellular C-terminal tail. Detection was performed using Alexa Fluor 488-conjugated antirabbit IgG (green) and Alexa Fluor 549-conjugated streptavidin (red). Cell nuclei were counterstained with Hoechst 33342 (blue). (A–F). Cells were fixed and permeabilized prior to simultaneous incubation with both the antibody and the linear polymer, allowing access to both surface and intracellular epitopes. (G–L). Cells were first incubated live with the N-terminal antibody, then fixed and permeabilized before incubation with the linear polymer. This approach restricted antibody binding to surface-exposed domains while permitting the polymer to access both compartments. CB 1 receptor-overexpressing cells (solid arrows) displayed strong signals for both probes, whereas nontransfected cells (open arrows) showed low or negligible labeling with the biotinylated linear polymer, supporting its specificity for the CB 1 receptor C-terminal region. Images correspond to maximum intensity projections of six consecutive optical sections (0.24 μm apart), acquired using structured illumination (ApoTome) on a Carl Zeiss Axio Observer microscope equipped with a motorized XYZ stage. Scale bar = 10 μm (applies to A–L).

    Techniques Used: Fluorescence, Labeling, Expressing, Polymer, Incubation, Binding Assay, Microscopy



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    Image Search Results


    Double fluorescence labeling of HEK293T cells transiently expressing the CB1 receptor using an anti-CB1 antibody and the biotinylated linear polymer fabricated here. Cells were colabeled with a rabbit polyclonal antibody targeting the extracellular N-terminal domain of the human CB 1 receptor and a biotinylated linear polymer specific for the last 12 amino acids of its intracellular C-terminal tail. Detection was performed using Alexa Fluor 488-conjugated antirabbit IgG (green) and Alexa Fluor 549-conjugated streptavidin (red). Cell nuclei were counterstained with Hoechst 33342 (blue). (A–F). Cells were fixed and permeabilized prior to simultaneous incubation with both the antibody and the linear polymer, allowing access to both surface and intracellular epitopes. (G–L). Cells were first incubated live with the N-terminal antibody, then fixed and permeabilized before incubation with the linear polymer. This approach restricted antibody binding to surface-exposed domains while permitting the polymer to access both compartments. CB 1 receptor-overexpressing cells (solid arrows) displayed strong signals for both probes, whereas nontransfected cells (open arrows) showed low or negligible labeling with the biotinylated linear polymer, supporting its specificity for the CB 1 receptor C-terminal region. Images correspond to maximum intensity projections of six consecutive optical sections (0.24 μm apart), acquired using structured illumination (ApoTome) on a Carl Zeiss Axio Observer microscope equipped with a motorized XYZ stage. Scale bar = 10 μm (applies to A–L).

    Journal: Analytical Chemistry

    Article Title: Controlled Photoiniferter RAFT-Based Development of Linear Polymers Induced by the Presence of a Peptide to Produce Synthetic Antibody Substitutes Applicable to Immunofluorescence Imaging Techniques

    doi: 10.1021/acs.analchem.5c05598

    Figure Lengend Snippet: Double fluorescence labeling of HEK293T cells transiently expressing the CB1 receptor using an anti-CB1 antibody and the biotinylated linear polymer fabricated here. Cells were colabeled with a rabbit polyclonal antibody targeting the extracellular N-terminal domain of the human CB 1 receptor and a biotinylated linear polymer specific for the last 12 amino acids of its intracellular C-terminal tail. Detection was performed using Alexa Fluor 488-conjugated antirabbit IgG (green) and Alexa Fluor 549-conjugated streptavidin (red). Cell nuclei were counterstained with Hoechst 33342 (blue). (A–F). Cells were fixed and permeabilized prior to simultaneous incubation with both the antibody and the linear polymer, allowing access to both surface and intracellular epitopes. (G–L). Cells were first incubated live with the N-terminal antibody, then fixed and permeabilized before incubation with the linear polymer. This approach restricted antibody binding to surface-exposed domains while permitting the polymer to access both compartments. CB 1 receptor-overexpressing cells (solid arrows) displayed strong signals for both probes, whereas nontransfected cells (open arrows) showed low or negligible labeling with the biotinylated linear polymer, supporting its specificity for the CB 1 receptor C-terminal region. Images correspond to maximum intensity projections of six consecutive optical sections (0.24 μm apart), acquired using structured illumination (ApoTome) on a Carl Zeiss Axio Observer microscope equipped with a motorized XYZ stage. Scale bar = 10 μm (applies to A–L).

    Article Snippet: After three washes with wash buffer, cells were incubated for 1 h with fluorescent secondary reagents Alexa Fluor 488-conjugated donkey antirabbit antibody (Invitrogen; 1:400) and DyLight 549-conjugated streptavidin (Vector Laboratories; 1:400) in blocking buffer.

    Techniques: Fluorescence, Labeling, Expressing, Polymer, Incubation, Binding Assay, Microscopy